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Tolonium chloride
IUPAC name (7-amino-8-methyl- phenothiazin-3-ylidene)- dimethyl-ammonium
Other names Toluidine blue O
Identifiers
CAS number [92-31-9]
PubChem 7084
MeSH Tolonium+chloride
SMILES CC1=CC2=C(C=C1N)SC3= CC(=[N+](C)C)C=CC3=N2
Properties
Molecular formula C15H16N3S+
Molar mass 270. IUPAC Nomenclature is a system of naming Chemical compounds and of describing the science of Chemistry in general CAS registry numbers are unique numerical identifiers for Chemical compounds Polymers biological sequences mixtures and Alloys They are also referred to PubChem is a Database of chemical Molecules The system is maintained by the National Center for Biotechnology Information (NCBI a component Medical Subject Headings ( MeSH) is a huge Controlled vocabulary (or metadata system for the purpose of indexing journal articles and books A chemical formula is a way of expressing information about the Atoms that constitute a particular Chemical compound, and how the relationship between those atoms changes Molar mass, symbol M, is the Mass of one mole of a substance ( Chemical element or Chemical compound) 374 g/mol
Except where noted otherwise, data are given for
materials in their standard state
(at 25 °C, 100 kPa)

Infobox disclaimer and references

Tolonium chloride (INN, also known as toluidine blue O) is a compound used in histology. In Chemistry, the standard state of a material is its state at 1 bar (100 Kilopascals exactly An International Nonproprietary Name ( INN; also known as rINN, for recommended International Nonproprietary Name or pINN for proposed Histology (from the Greek = 'tissue' is the study of the microscopic anatomy of cells and tissues of Plants and

Test for lignin

Toluidine blue solution is used in testing for lignin, a complex organic molecule that bonds to cellulose fibres and strengthens and hardens the cell walls in plants. In Chemistry, a solution is a Homogeneous Mixture composed of two or more substances Lignin or lignen is a complex Chemical compound most commonly derived from Wood and an integral part of the secondary Cell walls of Plants An organic compound is any member of a large class of Chemical compounds whose Molecules contain Carbon. Cellulose is an Organic compound with the formula, a Polysaccharide consisting of a linear chain of several hundred to over ten thousand β(1→4 A cell wall is a tough flexible and sometimes fairly rigid layer surrounding a cell, located external to the Cell membrane, which provides the cell with structural Plants are living Organisms belonging to the kingdom Plantae. A positive toluidine blue test causes the solution to turn from blue to blue-green. In Chemistry, a solution is a Homogeneous Mixture composed of two or more substances Blue is a Colour, the Perception of which is evoked by Green is a Color, the perception of which is evoked by light having a spectrum dominated by energy with a Wavelength of roughly 520–570- nm. A similar test can be performed with phloroglucinol-HCl solution, which turns red. Phloroglucinol or benzene-135-triol is a process chemical widely used in the industrial synthesis of pharmaceuticals and high-energy chemicals ( Explosives

Other Histological Uses

Toluidine Blue is often used to identify Mast cells. It is also used to stain proteoglycans and glycosaminoglycans in tissues such as cartilage. Proteoglycans represent a special class of Glycoproteins that are heavily glycosylated. Glycosaminoglycans (GAGs or mucopolysaccharides are long unbranched Polysaccharides consisting of a repeating Disaccharide unit


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