Silver staining is the use of silver to stain histologic sections. Silver (ˈsɪlvɚ is a Chemical element with the symbol " Ag " (argentum from the Ancient Greek: ἀργήντος - argēntos gen Histological section refers to thin slices of tissue applied to a Microscopic slide, usually around 5 to 10 Micrometres thick which are viewed This kind of staining is important especially to show proteins (for example type III collagen) and DNA. Proteins are large Organic compounds made of Amino acids arranged in a linear chain and joined together by Peptide bonds between the Carboxyl Collagen is the main Protein of Connective tissue in Animals and the most abundant protein in Mammals making up about 50% of the whole-body protein Deoxyribonucleic acid ( DNA) is a Nucleic acid that contains the genetic instructions used in the development and functioning of all known It is used to show both substances inside and outside cells. The cell is the structural and functional unit of all known living Organisms It is the smallest unit of an organism that is classified as living and is often called Silver staining is also used in temperature gradient gel electrophoresis and in polyacrylamide gels. Temperature Gradient Gel Electrophoresis ( TGGE) and Denaturing Gradient Gel Electrophoresis (DGGE are forms of Electrophoresis where there is a temperature
Some cells are argentaffin. These reduce silver solution to metallic silver after formalin fixation. Redox (shorthand for reduction-oxidation reaction describes all Chemical reactions in which atoms have their Oxidation number ( Oxidation state Formaldehyde is a Chemical compound with the formula H2CO It is the simplest Aldehyde —an organic compound containing a terminal Carbonyl In the fields of Histology, Pathology, and Cell biology, fixation is a chemical process by which Biological tissues are preserved from decay Other cells are argyrophilic. These reduce silver solution to metallic silver after being exposed to the stain that contains a reductant, for example hydroquinone or formalin. A reducing agent (also called a reductant or reducer) is the element or a compound in a Redox (reduction-oxidation reaction (see Electrochemistry Hydroquinone, also benzene-14-diol or quinol, is an Aromatic Organic compound which is a type of phenol, having the Chemical
Silver nitrate forms insoluble silver phosphate with phosphate ions. A phosphate, an Inorganic chemical, is a salt of Phosphoric acid. When subjected to a reducing agent, usually hydroquinone, it forms black elementary silver. Hydroquinone, also benzene-14-diol or quinol, is an Aromatic Organic compound which is a type of phenol, having the Chemical This is used for study of formation of calcium phosphate particles during bone growth. Calcium phosphate is the name given to a family of Minerals containing Calcium Ions (Ca2+ together with orthophosphates (PO43- Bones are rigid organs that form part of the Endoskeleton of Vertebrates They function to move support and protect the various organs of the body produce
Silver staining is used in light microscopy. Microscopy is the technical field of using microscopes to view samples or objects The metallic silver particles are deposited on sensitised reticulin fibres and are then easily seen in the microscopic preparations.
Silver stain aids in the perception of reticular fibers. Reticular fibers or reticulin is a histological term used to describe a type of Structural fiber composed of Type III collagen.
Use in art
Silver staining is also a technique in traditional stained glass to produce the yellow, brown or amber shading when painting on glass. For the Blackford Oakes novel see Stained Glass (novel The term stained glass refers either to the material of coloured Glass or to the art It is a technique that is often used for realistic hair colors. It was discovered in the 14th Century but was not originally used very frequently.
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