Nucleic acid methods are the techniques used to study Nucleic acids (DNA and RNA). A nucleic acid is a Macromolecule composed of chains of monomeric Nucleotides In Biochemistry these Molecules carry Genetic information Deoxyribonucleic acid ( DNA) is a Nucleic acid that contains the genetic instructions used in the development and functioning of all known Ribonucleic acid ( RNA) is a Nucleic acid that consists of a long chain of Nucleotide units
Purification
Quantification
- Abundance in weight: spectroscopic quantification
- Absolute abundance in number: RTPCR
- high-throughput relative abundance DNA microarray
- high-throughput absolute abundance SAGE
- Size: Gel electrophoresis
Synthesis
- De novo: Oligonucleotide synthesis
- Amplification: PCR
Other
- DNA sequencing
- Bisulfite sequencing
- Expression cloning
- Southern blot
- northern blot
- Sucrose gradient centrifugation
- Radioactivity in biological research
- Lab-on-a-chip
- Nuclear run-on assay
- Fluorescent in situ hybridization
- several Bioinformatics methods, such as RNA structure prediction
For protein methods, see Protein methods. Phenol-chloroform extraction is a Liquid-liquid extraction technique in Biochemistry. Column-based Nucleic acid Purification is a Solid phase extraction method to quickly purify Nucleic acids. Quantification of nucleic acids is commonly used in Molecular biology to determine the concentrations of DNA or RNA present in a mixture as subsequent In Molecular biology, real-time polymerase chain reaction, also called quantitative real time polymerase chain reaction (qPCR or kinetic polymerase chain reaction For terminology see glossary below A DNA microarray is a High-throughput technology used in Molecular biology and in Serial analysis of gene expression (SAGE is a technique used by molecular biologists to produce a snapshot of the Messenger RNA population in a sample of interest Oligonucleotide synthesis is the non-biological chemical synthesis of defined short sequences of Nucleic acids It is extremely useful in laboratory procedures covering a wide The term DNA sequencing encompasses biochemical methods for determining the order of the Nucleotide bases Adenine, Guanine, Cytosine Bisulfite sequencing is the use of Bisulfite treatment of DNA to determine its pattern of methylation Expression cloning is a technique in DNA cloning that uses Expression vectors to generate a library of clones with each clone expressing one protein A Southern blot is a method routinely used in Molecular biology to check for the presence of a DNA sequence in a DNA sample The northern blot is a technique used in Molecular biology research to study Gene expression. Sucrose gradient centrifugation is a type of Centrifugation often used to purify enveloped Viruses (with densities 1 Radioactivity can be used in life sciences as a Radiolabel to easily visualise components or target molecules in a biological system A lab-on-a-chip (LOC is a device that integrates one or several Laboratory functions on a single Chip of only millimeters to a few square centimeters in size A nuclear run-on assay is conducted to identify the Genes that are being transcribed at a certain time FISH ( Fluorescent In situ hybridization) is a cytogenetic technique that can be used to detect and localize the presence or absence Bioinformatics is the application of information technology to the field of molecular biology This list of RNA structure prediction software is a compilation of software tools and web portals used for RNA structure prediction Protein methods are the techniques used to study Proteins List of Protein Methods There are genetic methods for studying proteins methods for detecting proteins
- Protocols and other How-to information are not present in Wikipedia (see WP:NOT#HOWTO), for these one could consult Open wetware website
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