A process by which the genetic information of an organism is changed in a stable manner, either in nature or experimentally by the use of chemicals or radiation. A chemical substance is a Material with a definite chemical composition. Radiation, as in Physics, is Energy in the form of waves or moving Subatomic particles emitted by an atom or other body as it changes from a higher energy

Types Of Mutation

The following are the types of Mutagenesis:

• PCR Mutagenesis
• Transposon Mutagenesis
• Site-directed mutagenesis
• Directed mutagenesis
• Insertional mutagenesis
• Targeted mutagenesis

PCR Mutagenesis

PCR mutagenesis is simple method for generating Site-directed mutagenesis. Transposon mutagenesis or transposition mutagenesis is a biology process that allows genes to be transferred to a host organism's Chromosome, interrupting or modifying Site-directed Mutagenesis is a Molecular biology technique in which a Mutation is created at a defined site in a DNA molecule usually a circular Directed mutagenesis, also known as directed mutation, is a Hypothesis proposing that organisms can respond to environmental stresses through directing mutations to Insertional mutagenesis is Mutagenesis of dna by the insertion of one or more bases Site-directed Mutagenesis is a Molecular biology technique in which a Mutation is created at a defined site in a DNA molecule usually a circular Site-directed Mutagenesis is a Molecular biology technique in which a Mutation is created at a defined site in a DNA molecule usually a circular This method can generate mutations (base substitutions, insertions, and deletions) from double-stranded plasmid without the need for subcloning into M13-based bacteriophage vectors and for ssDNA rescue. This article is about a biological infectious particle for other uses see Phage (disambiguation. The procedure involves a PCR reaction using a supercoiled plasmid vector as the template and two synthetic oligonucleotide primers containing the desired mutation with each complementary to the opposite strands of the vector. After PCR, the template (wild type) plasmid which is dam methylated in almost all E. coli is removed by digestion with Dpn I which is specific for methylated DNA. The mutated plasmid remains intact in the reaction.

Also See

• Mutation

In biology mutations are changes to the Nucleotide sequence of the Genetic material of an organism

© 2009 citizendia.org; parts available under the terms of GNU Free Documentation License, from http://en.wikipedia.org
Dapyx Software network: MP3 Explorer | Ebook Manager | Zenithic