Gp120 - Citizendia

gp120

gp120 is a glycoprotein exposed on the surface of the HIV envelope. Not to be confused with Peptidoglycan. Glycoproteins are proteins that contain Oligosaccharide chains ( Glycans) covalently attached The Genome and Proteins of HIV have been the subject of extensive research since the discovery of the virus in 1983 The 120 in its name comes from its molecular weight of 120 kilodaltons. The molecular mass (abbreviated m of a substance, more commonly referred to as molecular weight and abbreviated as MW, is the Mass of one The unified atomic mass unit ( u) or Dalton ( Da) or sometimes universal mass unit, is an unit of Mass used to express

The crystal structure of gp120 complexed to D1D2 CD4 and a neutralizing antibody Fab was solved by Peter Kwong in 1998. It is organized with an outer domain, an inner domain with respect to its termini and a bridging sheet. The β sheet (also β-pleated sheet) is the second form of regular Secondary structure in Proteins consisting of beta strands connected laterally The gp120 gene is around 1. 5Kb long and codes for around 500 amino acids. Three copies of gp120 form into a trimer that caps the end of gp41. gp41 is a Glycoprotein non- covalently -bound to gp120 and provides the second step by which HIV enters the cell

The Human Immunodeficiency Virus (HIV) can mutate frequently to stay ahead of the immune system. Human immunodeficiency virus ( HIV) is a Lentivirus (a member of the Retrovirus family that can lead to acquired immunodeficiency syndrome An immune system is a collection of mechanisms within an Organism that protects against Disease by identifying and killing Pathogens and Tumor There is however a highly conserved region in the virus genome near its receptor binding site. The glycoprotein gp120 is anchored to the viral membrane through non-covalent bonds along with gp41, both coming from a cleaved protein, gp160. Many Viruses (eg influenza and many animal viruses have viral envelopes covering their protein Capsids The envelopes are typically derived from portions of gp41 is a Glycoprotein non- covalently -bound to gp120 and provides the second step by which HIV enters the cell The Genome and Proteins of HIV have been the subject of extensive research since the discovery of the virus in 1983 It infects any target cell with a CD4 receptor, particularly the helper T-cell, by binding to that receptor. CD4 ( Cluster of differentiation 4 is a Glycoprotein expressed on the surface of T helper cells, Regulatory T cells, Monocytes, In Biochemistry, a receptor is a Protein molecule embedded in either the Plasma membrane or Cytoplasm of a cell to which a mobile signaling T helper cells (also known as effector T cells or Th cells) are a sub-group of Lymphocytes (a type of White blood cell or Binding to CD4 is mainly electrostatic although there are van der Waals interactions and hydrogen bonds. Electrostatics is the branch of Science that deals with the Phenomena arising from what seems to be stationary Electric charges Since Classical A hydrogen bond results from a Dipole-dipole force between an Electronegative atom and a Hydrogen atom bonded to Nitrogen, Oxygen

The exact mechanism of virus entry into a cell is unknown. However, gp120 plays a vital role in seeking out receptors on host cells for entry.

1 gp120 vaccines
2 Competition
3 References
4 See also

gp120 vaccines

Since CD4 receptor binding is the most obvious step in HIV infection, gp120 was among the first targets of HIV vaccine research. An HIV vaccine is a hypothetical Vaccine against HIV, the etiological agent of AIDS. These efforts have been hampered by the chemical and structural properties of gp120, which make it difficult for antibodies to bind to it; also, it can easily be shed from the virus' surface and captured by T-cells due to its loose binding with gp41. Antibodies (also known as immunoglobulins, abbreviated Ig) are Gamma globulin Proteins that are found in Blood or other Bodily T cells belong to a group of White blood cells known as Lymphocytes, and play a central role in Cell-mediated immunity. This has traditionally been a hindrance in developing gp120 based HIV vaccines. However, a study published in the February 15, 2007 edition of Nature provides new promise in the field ^[1]. The researchers have identified a region of susceptibilty in the gp120 glycoprotein that corresponds to a functional requirement for efficient association with CD4. This conserved region is involved in the metastable attachment of gp120 to CD4, and can potentially be targeted by the broadly neutralizing antibody b12. This study is promising in its prospects for developing a vaccine targeted at the invariant surface.

Competition

Main article: Entry inhibitors

The protein gp120 is necessary during the initial binding of HIV to its target cell. Consequently, anything which binds to gp120's target can block gp120 from binding to a cell by being physically in the way. Many of these are toxic to the immune system, such as the anti-CD4 monoclonal antibody OKT4.

EGCG, a flavonoid found in green tea, binds to the same CD4 receptor that gp120 binds to, effectively competing for this receptor. Green tea is a type of Tea made solely with the leaves of Camellia sinensis, that has undergone minimal Oxidation during processing Test tube studies suggested that EGCG concentrations as low as 0. 2 mmols/L – the amount of the molecule found in a cup or two of green tea – temporarily reduced HIV-CD4 cell binding by 40%. ^[2] Substantial further research is needed both to confirm that this one-time laboratory experiment can be repeated successfully, and also to see whether it has any practical effect. For example, by binding to CD4, the flavonoid might slow HIV disease progression in a patient, but it might also attract an immune system-destroying antibody response against the non-human flavonoid.

References

^ Zhou, Tongqing et al. Structural definition of a conserved neutralization epitope on HIV-1 gp120. Nature, 2007. Vol 445, 15 February 2007, pp 732-737
^ AIDSmeds.com - Top Stories : Green Tea for HIV? - by Tim Horn

Contents

gp120 vaccines

Competition

References

See also