Eosin is a fluorescent red dye resulting from the action of bromine on fluorescein. Fluorescence is a Luminescence that is mostly found as an A dye can generally be described as a Colored substance that has an affinity to the substrate to which it is being applied Fluorescein is a Fluorophore commonly used in Microscopy, in a type of Dye laser as the Gain medium, in Forensics and Serology It can be used to stain cytoplasm, collagen and muscle fibers for examination under the microscope. The cytoplasm is the contents of a cell that is enclosed within the Plasma membrane. Collagen is the main Protein of Connective tissue in Animals and the most abundant protein in Mammals making up about 50% of the whole-body protein Muscle (from Latin musculus, diminutive of mus "mouse" is contractile tissue of the body and is derived from the A microscope ( Greek: ( micron) = small + ( skopein) = to look or see is an instrument for viewing objects that are
There are actually two very closely related compounds commonly referred to as eosin. Most often used is eosin Y (also known as eosin Y ws, eosin yellowish, Acid Red 87, C. I. 45380, bromoeosine, bromofluoresceic acid, D&C Red No. 22); it has a very slightly yellowish cast. The other eosin compound is eosin B (eosin bluish, Acid Red 91, C. I. 45400, Saffrosine, Eosin Scarlet, or imperial red); it has a very faint bluish cast. The two dyes are interchangeable, and the use of one or the other is a matter of preference and tradition.
Eosin is most often used as a counterstain to haematoxylin in H&E (haematoxylin and eosin) staining. A counterstain is a stain with color contrasting to the principal stain making the stained structure more easily visible Haematoxylin, hematoxylin, Natural Black 1, or CI 75290 is extracted from the wood of the Logwood tree H&E stain, HE stain or hematoxylin and eosin stain, is a popular Staining method in Histology. H&E staining is one of the most commonly used techniques in histology. Histology (from the Greek = 'tissue' is the study of the microscopic anatomy of cells and tissues of Plants and Tissue stained with hematoxylin and eosin shows cytoplasm stained pink-orange and nuclei stained darkly, either blue or purple. Tissue is a cellular organizational level intermediate between cells and a complete organism Haematoxylin, hematoxylin, Natural Black 1, or CI 75290 is extracted from the wood of the Logwood tree The cytoplasm is the contents of a cell that is enclosed within the Plasma membrane. In Cell biology, the nucleus (pl nuclei; from Latin la ''nucleus'' or la ''nuculeus'' "little nut" or kernel is a membrane-enclosed Eosin also stains red blood cells intensely red. Red blood cells are the most common type of Blood cell and the Vertebrate body's principal means of delivering Oxygen to the body tissues via the Blood Eosin is an acidic dye and shows up in the basic parts of the cell, ie the cytoplasm. Hematoxylin however is a basic dye and shows up in the acidic part of the cell. Haematoxylin, hematoxylin, Natural Black 1, or CI 75290 is extracted from the wood of the Logwood tree For example the nucleus, where nucleic acids (DNA and RNA) are concentrated. A nucleic acid is a Macromolecule composed of chains of monomeric Nucleotides In Biochemistry these Molecules carry Genetic information Deoxyribonucleic acid ( DNA) is a Nucleic acid that contains the genetic instructions used in the development and functioning of all known Ribonucleic acid ( RNA) is a Nucleic acid that consists of a long chain of Nucleotide units
Structures that stain readily with eosin are termed eosinophilic. See also Eosin Eosinophilic means loves eosin, and refers to the Staining of certain tissues, cells, or Organelles
Eosin Y is a tetrabromo derivate of fluorescein. Fluorescein is a Fluorophore commonly used in Microscopy, in a type of Dye laser as the Gain medium, in Forensics and Serology Its CAS number is [] and its SMILES structure is O=C5C(Br)=C2O C1=C(Br)C([O-]) =C(Br)C=C1C(C4=C (C([O-])=O)C=C C=C4)=C2C=C3Br. CAS registry numbers are unique numerical identifiers for Chemical compounds Polymers biological sequences mixtures and Alloys They are also referred to
Eosin B is a dibromo dinitro derivate of fluorescein. Fluorescein is a Fluorophore commonly used in Microscopy, in a type of Dye laser as the Gain medium, in Forensics and Serology Its CAS number is [] and its SMILES structure is O=C5C(Br)=C2O C1=C(Br)C([O-]) =C([N+]([O-])=O) C=C1C(C4=C(C([O-]) =O)C=CC=C4)=C2 C=C3[N+]([O-])=O. CAS registry numbers are unique numerical identifiers for Chemical compounds Polymers biological sequences mixtures and Alloys They are also referred to
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Recipes for Histology
5% Aqueous Eosin Y
Formula:
- 5 g Eosin Y
- 100 mL Distilled water
Dissolve Eosin Y crystals in water by gentle heating. Histology (from the Greek = 'tissue' is the study of the microscopic anatomy of cells and tissues of Plants and Cool and Filter. Thymol crystals may be added to prevent formation of molds. Thymol is a Monoterpene phenol derivative of Cymene, C10H14OH Isomeric with Carvacrol, found in oil of
Eosin, Stock Alcoholic Solution
Formula:
- 1 g Eosin Y
- 20 mL Distilled water
- 95% Alcohol
Dissolve Eosin Y in the water by gentle heating. Cool and add Alcohol.
For use, one part of the stock solution is usually diluted with three parts of 80% Alcohol. Addition of 0. 5 mL Glacial Acetic Acid (HOAc) for every 100 mL of stain will usually give a deeper red stain to the tissue. Acetic acid, also known as ethanoic acid, is an organic chemical compound, giving Vinegar its sour taste
Etymology
The name Eosin comes from Eos, the Greek word for 'dawn' and the name of the Greek Goddess of the Dawn. For other uses of the name Eos see Eos (disambiguation. For the Slavic goddesses called the Auroras see The Zorya. [1]
See also
External links
References
- Jocelyn H. Haematoxylin, hematoxylin, Natural Black 1, or CI 75290 is extracted from the wood of the Logwood tree H&E stain, HE stain or hematoxylin and eosin stain, is a popular Staining method in Histology. Bruce-Gregorios, M. D. : Histopathologic Techniques, JMC Press Inc. , Quezon City, Philippines, 1974.
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