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DCPIP
DCPIP
IUPAC name 2,6-dichlorophenol-indophenol
Other names 2,5-cyclohexadien-1-one;

dichloroindophenol;
2,6-dichloro-4-[(4-hydroxyphenyl)imino] -2,6-dichloroindophenol;
2,6-Dichloro-4-((4-hydroxyphenyl)imino) -2,5-cyclohexadien-1-one
Identifiers
CAS number [956-48-9]
SMILES O=C(C(Cl)=C2)C(Cl)=C/ C2=N\C1=CC=C(O)C=C1
Properties
Molecular formula C12H7NCl2O2
Molar mass 268. IUPAC Nomenclature is a system of naming Chemical compounds and of describing the science of Chemistry in general CAS registry numbers are unique numerical identifiers for Chemical compounds Polymers biological sequences mixtures and Alloys They are also referred to A chemical formula is a way of expressing information about the Atoms that constitute a particular Chemical compound, and how the relationship between those atoms changes Molar mass, symbol M, is the Mass of one mole of a substance ( Chemical element or Chemical compound) 1 g mol−1
Except where noted otherwise, data are given for
materials in their standard state
(at 25 °C, 100 kPa)
Infobox disclaimer and references

2,6-dichlorophenolindophenol, ( DCPIP ) is a blue chemical compound used as a redox dye. In Chemistry, the standard state of a material is its state at 1 bar (100 Kilopascals exactly A chemical compound is a substance consisting of two or more different elements chemically bonded together in a fixed proportion by Mass. A redox indicator (also called an oxidation-reduction indicator) is an Indicator that undergoes a definite color change at a specific Electrode potential Oxidized DCPIP is blue, reduced DCPIP is colorless. Redox (shorthand for reduction-oxidation reaction describes all Chemical reactions in which atoms have their Oxidation number ( Oxidation state

The rate of photosynthesis can be measured by the rate at which this dye is broken down (reduced) when exposed to light in a photosynthetic system. This reaction is reversible, as colorless DCPIP can be reoxidized to blue. It is often used in measurements of the electron transport chain in plants because of its higher [affinity] for electrons than ferredoxin. An electron transport chain couples a chemical reaction between an electron donor (such as NADH) and an electron acceptor (such as O2) to the transfer Ferredoxins (from Latin ferrum: Iron + Redox, often abbreviated "fd" are Iron-sulfur proteins that mediate Electron For example, it is possible to compare rates of photosynthesis with DCPIP. Photosynthesis is a Metabolic pathway that converts Light Energy into Chemical energy. Due to the reductions that occur during the light reactions, DCPIP can be substituted for NADPH, the final electron carrier in the light reactions. Nicotinamide adenine dinucleotide phosphate ( NADP+, in older notation triphosphopyridine nucleotide TPN) is used in anabolic reactions such as Lipid The light reactions will reduce the DPIP and turn it colorless. The initial stage of the photosynthetic system is the light-dependent reaction, which converts solar energy into Potential energy. As DCPIP is reduced and becomes colorless, the following increase in light transmittance can be measured by a spectrophotometer. In Physics, spectrophotometry is the quantitative study of electromagnetic spectra.

The reduction of DCPIP

DCPIP

DCPIP is a redox dye commonly used as a monitor of the light reactions in photosynthesis because it is an electron acceptor that is blue when oxidized and colourless when reduced. A redox indicator (also called an oxidation-reduction indicator) is an Indicator that undergoes a definite color change at a specific Electrode potential The initial stage of the photosynthetic system is the light-dependent reaction, which converts solar energy into Potential energy. Photosynthesis is a Metabolic pathway that converts Light Energy into Chemical energy. The electron is a fundamental Subatomic particle that was identified and assigned the negative charge in 1897 by J Redox (shorthand for reduction-oxidation reaction describes all Chemical reactions in which atoms have their Oxidation number ( Oxidation state It is part of the Hill reagents family. Discovered in 1937 by Robin Hill, these reagents allowed the discovery of electron transport chains during Photosynthesis. DPIP is commonly used as a substitute for NADP+. Nicotinamide adenine dinucleotide, abbreviated NAD+, is a Coenzyme found in all living cells The compound is a dinucleotide since it consists The dye changes color when it is reduced, due to its chemical structure. Chemical structure refers to Molecular geometry, Electronic structure and Crystal structure. The nitrogen atom in the center of the molecule is the atom that accepts electrons, and it changes the double N-C bond to a single bond, which forces bonds between carbons in the entire left ring to change. Nitrogen (ˈnaɪtɹəʤɪn is a Chemical element that has the symbol N and Atomic number 7 and Atomic weight 14 This microscopic shift in the DCPIP structure causes the macroscopic change in colour, from dark blue to colourless. Macroscopic is commonly used to describe physical objects that are measurable and observable by the Naked eye.

The rate of photosynthesis light-dependent reaction can be measured with this property of DCPIP, because one of the stages of the light reaction is an electron transport chain that normally ends with the reduction of NADP+. An electron transport chain couples a chemical reaction between an electron donor (such as NADH) and an electron acceptor (such as O2) to the transfer When DCPIP is present, it also gets reduced by the light reaction. The amount of DCPIP reduced can be found by measuring the solution's light transmittance with a spectrophotometer. In Optics and Spectroscopy, transmittance is the fraction of incident light at a specified Wavelength that passes through a sample In Physics, spectrophotometry is the quantitative study of electromagnetic spectra.

Using DCPIP to determine the concentration of ascorbic acid or Vitamin C in a solution. In Chemistry, concentration is the measure of how much of a given substance there is mixed with another substance This article deals with the molecular aspects of ascorbic acid Vitamin C or L-ascorbate is an Essential nutrient for a large number of higher primate species a small number of other Mammalian

DCPIP is commonly used as an indicator for Vitamin C. Vitamin C or L-ascorbate is an Essential nutrient for a large number of higher primate species a small number of other Mammalian If vitamin C, which is a good reducing agent is present , the blue dye, which turns pink in acid conditions and is reduced to a colourless compound by ascorbic acid.

DCPIP (blue) + H+ ----------> DCPIPH (pink)
DCPIPH (pink) + VitC ----------> DCPIPH2 (colourless)
C6H8O6 + C12H7NCl2O2 ----------> C6H6O6 + C12H9NCl2O2

In a titration, when all the ascorbic acid in the solution has been used up, there will not be any electrons available to reduce the DCPIPH and the solution will remain pink due to the DCPIPH. The end point is a pink colour that persists for 10 seconds or more.

References

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