| Auramine O | |
|---|---|
 |
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| IUPAC name | bis[4-(dimethylamino)phenyl]methaniminium chloride |
| Identifiers | |
| CAS number | [2465-27-2] |
| SMILES | [NH2+]=C (C2=CC=C (N(C)C)C=C2) C1=CC=C(N (C)C)C=C1 |
| Properties | |
| Molecular formula | C17H22N3Cl |
| Molar mass | 303. IUPAC Nomenclature is a system of naming Chemical compounds and of describing the science of Chemistry in general CAS registry numbers are unique numerical identifiers for Chemical compounds Polymers biological sequences mixtures and Alloys They are also referred to A chemical formula is a way of expressing information about the Atoms that constitute a particular Chemical compound, and how the relationship between those atoms changes Molar mass, symbol M, is the Mass of one mole of a substance ( Chemical element or Chemical compound) 8339 g/mol |
| Melting point |
267 °C |
| Hazards | |
| R-phrases | R22 R24 R40 |
| S-phrases | S36/37 S45 |
| Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox disclaimer and references |
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Auramine O, also called Basic yellow 2, Pyocatanium aureum, aizen auramine, Pyoktanin Yellow, Canary Yellow, Pyoktanin, or C. The melting point of a solid is the temperature range at which it changes state from solid to Liquid. R-phrases (short for Risk Phrases) are defined in Annex III of European Union Directive 67/548/EEC: Nature of special risks attributed to dangerous R-phrases (short for Risk Phrases) are defined in Annex III of European Union Directive 67/548/EEC: Nature of special risks attributed to dangerous R-phrases (short for Risk Phrases) are defined in Annex III of European Union Directive 67/548/EEC: Nature of special risks attributed to dangerous R-phrases (short for Risk Phrases) are defined in Annex III of European Union Directive 67/548/EEC: Nature of special risks attributed to dangerous S-phrases are defined in Annex IV of European Union Directive 67/548/EEC: Safety advice concerning dangerous substances and preparations. S-phrases are defined in Annex IV of European Union Directive 67/548/EEC: Safety advice concerning dangerous substances and preparations. S-phrases are defined in Annex IV of European Union Directive 67/548/EEC: Safety advice concerning dangerous substances and preparations. In Chemistry, the standard state of a material is its state at 1 bar (100 Kilopascals exactly I. 41000, is a diarylmethane dye used as a fluorescent stain. A dye can generally be described as a Colored substance that has an affinity to the substrate to which it is being applied Staining is an auxiliary Technique used in Microscopy to enhance contrast in the microscopic image In its pure form, Auramine O appears as yellow needle crystals. It is very soluble in water and soluble in ethanol.
Auramine O can be used to stain acid-fast bacteria (e. Acid-fastness is a physical property of some bacteria referring to their resistance to decolorization by acids during staining procedures The Bacteria ( singular: bacterium) are a large group of unicellular Microorganisms Typically a few Micrometres in length bacteria have g. Mycobacterium), where it binds to the mycolic acid in its cell wall) in a way similar to Ziehl-Neelsen stain. Mycobacterium is a Genus of Actinobacteria, given its own family the Mycobacteriaceae Mycolic acids are long Fatty acids found in the Cell walls of the mycolata Taxon, a group of Bacteria that includes Mycobacterium A cell wall is a tough flexible and sometimes fairly rigid layer surrounding a cell, located external to the Cell membrane, which provides the cell with structural The Ziehl-Neelsen stain, also known as the acid-fast stain, was first described by two German doctors Franz Ziehl (1859 to 1926 a bacteriologist and Friedrich [1] It can also be used as a fluorescent version of Schiff reagent. The Schiff test invented and named after Hugo Schiff is a Chemical test for the detection of Aldehydes. [2]
Auramine O can be used together with Rhodamine B as the Truant auramine-rhodamine stain for Mycobacterium tuberculosis. Rhodamine (ˈroʊdəmiːn is a family of related chemical compounds Fluorone dyes The auramine-rhodamine stain (AR also known as the Truant auramine-rhodamine stain, is a histological technique used to visualizee acid-fast bacilli Mycobacterium tuberculosis is the Bacterium that causes most cases of Tuberculosis. [3][4] It can be also used as an antiseptic agent. Antiseptics (from Greek αντί - anti, '"against" + σηπτικός - septikos, "putrefactive" are antimicrobial
References
- ^ Kommareddi S, Abramowsky C, Swinehart G, Hrabak L (1984). "Nontuberculous mycobacterial infections: comparison of the fluorescent auramine-O and Ziehl-Neelsen techniques in tissue diagnosis". Hum Pathol 15 (11): 1085–9. doi:. A digital object identifier ( DOI) is a permanent identifier given to an Electronic document. PMID 6208117.
- ^ Khavkin T, Kudryavtseva M, Dragunskaya E, Polotsky Y, Kudryavtsev B (1980). "Fluorescent PAS-reaction study of the epithelium of normal rabbit ileum and after challenge with enterotoxigenic Escherichia coli". Gastroenterology 78 (4): 782–90. PMID 6986320.
- ^ Truant J, Brett W, Thomas W (1962). "Fluorescence microscopy of tubercle bacilli stained with auramine and rhodamine". Henry Ford Hosp Med Bull 10: 287–96. PMID 13922644.
- ^ Arrowood M, Sterling C (1989). "Comparison of conventional staining methods and monoclonal antibody-based methods for Cryptosporidium oocyst detection". J Clin Microbiol 27 (7): 1490–5. PMID 2475523.
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